Anti-CDw210a/IL10RA Polyclonal Antibody (HC614014)

Name: Anti-CDw210a/IL10RA Polyclonal Antibody
Brand: abinScience
SKU: HC614014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC614014

Description

Anti-CDw210a/IL10RA Polyclonal Antibody (HC614014) is a rabbit polyclonal antibody detecting CDw210a in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CDw210a/IL10RA (Leu132-Asn235).

Target

IL-10 receptor subunit alpha, IL-10R1, Interleukin-10 receptor subunit alpha, Interleukin-10 receptor subunit 1, CD210, IL10RA, CDw210a, IL-10R subunit alpha, IL-10R subunit 1, IL10R, IL-10RA

Purification

Purified by antigen affinity column.

Accession

Q13651

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-10 receptor subunit alpha (CDw210a/IL10RA) is a ~63 kDa protein. Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes.

1. Yoon, SI. et al. (2006) The Journal of biological chemistry 281, 35088-96. PMID: 16982608

2. Usacheva, A. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 169, 1302-8. PMID: 12133952

3. Shi, J. et al. (2016) Cell death & disease 7, e2133. PMID: 26962683

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CDw210a/IL10RA polyclonal antibody at 1ug/mL on recombinant CDw210a/IL10RA (Catalog No. HC614012).

References

Datasheet