Anti-CEBPA Polyclonal Antibody (HW834024)

Name: Anti-CEBPA Polyclonal Antibody
Brand: abinScience
SKU: HW834024
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HW834024

Description

Anti-CEBPA Polyclonal Antibody (HW834024) is a rabbit polyclonal antibody detecting CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; CEBP in ELISA, IHC, WB. Suitable for Mouse, Bovine, Rat, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human CEBPA (Asn281-Ala358).

Target

CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; CEBP

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P53566, O02754, P05554, P49715

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CCAAT/enhancer-binding protein alpha (CEBPA) is a ~37 kDa protein. Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes. During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung.

1. Wang, GL. et al. (2004) Genes & development 18, 912-25. PMID: 15107404

2. Zhang, P. et al. (2004) Immunity 21, 853-63. PMID: 15589173

3. Zhang, Z. et al. (2022) Cell metabolism 34, 1860-1874.e4. PMID: 36228616

4. Lin, FT. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9606-10. PMID: 8415748

5. Bégay, V. et al. (2004) Molecular and cellular biology 24, 9744-51. PMID: 15509779

6. Ohlsson, E. et al. (2014) The Journal of experimental medicine 211, 5-13. PMID: 24367003

7. Flodby, P. et al. (1996) The Journal of biological chemistry 271, 24753-60. PMID: 8798745

8. Samuelsson, L. et al. (1991) The EMBO journal 10, 3787-93. PMID: 1935900

9. Lin, FT. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 8757-61. PMID: 8090719

10. Porse, BT. et al. (2001) Cell 107, 247-58. PMID: 11672531

Note

For research use only

Images

References

Datasheet

Anti-CEBPA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-CEBPA Polyclonal Antibody (HW834024)

Overview

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References

Datasheet

SDS

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