Anti-CFL1 Polyclonal Antibody (HB213014)

Name: Anti-CFL1 Polyclonal Antibody
Brand: abinScience
SKU: HB213014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB213014

Description

Anti-CFL1 Polyclonal Antibody (HB213014) is a rabbit polyclonal antibody detecting CFL1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CFL1 (Ser3-Leu161).

Target

Cofilin, non-muscle isoform, CFL1, p18, 18 kDa phosphoprotein, CFL, Cofilin-1

Purification

Purified by antigen affinity column.

Accession

P23528

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cofilin-1 (CFL1) is a ~18 kDa protein. Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

1. Yeoh, S. et al. (2002) Journal of molecular biology 315, 911-25. PMID: 11812157

2. Gohla, A. et al. (2005) Nature cell biology 7, 21-9. PMID: 15580268

3. Bai, SW. et al. (2011) BMC biology 9, 54. PMID: 21834987

4. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CFL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: H1299 cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Rat brain lysate

References

Datasheet

Anti-CFL1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CFL1 Polyclonal Antibody (HB213014)

Overview

Images

References

Datasheet

SDS

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