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Anti-cGMP Monoclonal Antibody (1A428) (YA864015)

Anti-cGMP Monoclonal Antibody (1A428)
Anti-cGMP Monoclonal Antibody (1A428)
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Overview
Catalog No.YA864015
Description
Anti-cGMP Monoclonal Antibody (1A428) [1A428] (YA864015) is a mouse monoclonal antibody detecting cGMP in ELISA, IHC, WB.
Highlights
  • ●Multi-Application — Validated across multiple applications.
Species reactivityGeneral
ApplicationsELISA, IHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG
Clone ID1A428
Immunogen cGMP
Target cGMP, cyclic guanosine monophosphate, cyclic GMP
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession CAS: 7665-99-8
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Guanosine 3’, 5’-cyclic monophosphate (cGMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cGMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cGMP ELISA Kit is based on the competition between HRP-labeled cGMP and free cGMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cGMP MAb) binding sites. Because the concentration of the HRP-labeled cGMP is held constant while the concentration of cGMP varies, the amount of HRP-labeled cGMP that is able to bind to the cGMP MAb will be inversely proportional to the concentration of cGMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50 (50% B/B0) of approximately 5 pmol/mL and a detection limit of approximately 0.1 pmol/mL.

Note For research use only.
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  • Anti-cGMP Monoclonal Antibody (1A428)

    SDS-PAGE

    SDS-PAGE for Anti-cGMP Monoclonal Antibody (1A428)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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