

| Catalog No. | YA864015 |
|---|---|
| Description |
Anti-cGMP Monoclonal Antibody (1A428) [1A428] (YA864015) is a mouse monoclonal antibody detecting cGMP in ELISA, IHC, WB.
Highlights
|
| Species reactivity | General |
| Applications | ELISA, IHC, WB |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone ID | 1A428 |
| Immunogen | cGMP |
| Target | cGMP, cyclic guanosine monophosphate, cyclic GMP |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | CAS: 7665-99-8 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Background | Guanosine 3’, 5’-cyclic monophosphate (cGMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cGMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cGMP ELISA Kit is based on the competition between HRP-labeled cGMP and free cGMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cGMP MAb) binding sites. Because the concentration of the HRP-labeled cGMP is held constant while the concentration of cGMP varies, the amount of HRP-labeled cGMP that is able to bind to the cGMP MAb will be inversely proportional to the concentration of cGMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50 (50% B/B0) of approximately 5 pmol/mL and a detection limit of approximately 0.1 pmol/mL. |
| Note | For research use only. |

SDS-PAGE for Anti-cGMP Monoclonal Antibody (1A428)




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