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Anti-CHIKV NSP1 Polyclonal Antibody (VK640024)

Anti-CHIKV NSP1 Polyclonal Antibody
Anti-CHIKV NSP1 Polyclonal Antibody
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Overview
Catalog No.VK640024
Description
Anti-CHIKV NSP1 Polyclonal Antibody (VK640024) is a rabbit polyclonal antibody detecting mRNA-capping enzyme nsP1 in ELISA, IHC, WB. Suitable for Chikungunya virus and O'nyong-nyong virus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityChikungunya virus (CHIKV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant CHIKV Polyprotein P1234 (Met1-Gln364).
Target Polyprotein P1234, P1234, Non-structural polyprotein, Polyprotein P123, P123, mRNA-capping enzyme nsP1, EC:2.1.1.-, EC:2.7.7.-, Non-structural protein 1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q8JUX6, Q5XXP4, O90368, P13886, O90370
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Polyprotein P1234 (nsp1) is a ~275 kDa protein. Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Note For research use only
Images
  • Anti-CHIKV NSP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NSP1-CHPV polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant CHIKV NSP1 (Catalog No: VK640022)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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