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Anti-CHPV M/Matrix protein Polyclonal Antibody (VK535014)

Anti-CHPV M/Matrix protein Polyclonal Antibody
Anti-CHPV M/Matrix protein Polyclonal Antibody
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Overview
Catalog No.VK535014
Description
Anti-CHPV M/Matrix protein Polyclonal Antibody (VK535014) is a rabbit polyclonal antibody detecting Matrix protein in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityChandipura virus (CHPV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Chandipura virus (CHPV) M/Matrix protein (Phe47-His229).
Target Matrix protein, M
Purification Purified by antigen affinity column.
Accession Q9WH76
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Matrix protein is a ~26 kDa protein. Forms a double layer around the helical nucleocapsid, the inner matrix layer binding to the N helix and the outer matrix layer binding to the envelope glycoprotein. Plays a major role in assembly and budding of virion, by recruiting cellular partners of the ESCRT complexes that play a key role in releasing the budding particle from the host membrane. Condensates the ribonucleocapsid core during virus assembly. Inhibits the host mRNA nuclear export thereby inducing the shut off of cellular transcription and preventing the interferon signaling and the establishment of antiviral state in infected cells. This shutoff presumably inhibits interferon signaling and thus establishment of antiviral state in virus infected cells.

Note For research use only.
Images
  • Anti-CHPV M/Matrix protein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Matrix-CHPV polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant CHPV M/Matrix protein (Catalog No: VK535012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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