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Anti-CLIC1 Polyclonal Antibody (HT459014)

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Overview
Catalog No.HT459014
Description
Anti-CLIC1 Polyclonal Antibody (HT459014) is a rabbit polyclonal antibody detecting CLIC1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CLIC1 (Lys79-Lys241).
Target Regulatory nuclear chloride ion channel protein, CLIC1, NCC27, Nuclear chloride ion channel 27, Chloride channel ABP, Chloride intracellular channel protein 1, hRNCC, G6
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O00299
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Chloride intracellular channel protein 1 (CLIC1) is a ~26 kDa protein. In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Reduces selenite and dehydroascorbate and may act as an antioxidant during oxidative stress response. Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Involved in regulation of the cell cycle.

1. Al Khamici, H. et al. (2015) PloS one 10, e115699. PMID: 25581026
2. Alghalayini, A. et al. (2023) Biomolecules 13. PMID: 37759794
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CLIC1 Polyclonal Antibody

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