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Anti-Clostridium botulinum botA/BOTOXPolyclonal Antibody (JN888014)

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Overview
Catalog No.JN888014
Description
Anti-Clostridium botulinum BotA Polyclonal Antibody (JN888014) is a rabbit polyclonal antibody detecting botA. Suitable for Clostridium botulinum.
Species reactivityClostridium botulinum
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Clostridium botulinum BotA (Met1-Glu424).
Target BOTOX, BoNT/A, Bontoxilysin-A, Botulinum neurotoxin A heavy chain, Botulinum neurotoxin A light chain, Botulinum neurotoxin type A, Botulinum neurotoxin type A1, EC:3.4.24.69, HC, LC, atx, bonT, botA
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P0DPI0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Botulinum neurotoxin type A (BotA) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302
2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132
3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397
4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517
5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155
6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415
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8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274
Note For research use only
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Formula
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Anti-Clostridium botulinum botA/BOTOXPolyclonal Antibody

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