Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014)

Name: Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody
Brand: abinScience
SKU: JN055014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
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Overview

Catalog No.

JN055014

Description

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Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014) is a rabbit polyclonal antibody detecting Toxin A in ELISA, IHC, WB. Suitable for Clostridium novyi.

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Highlights

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●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

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Species reactivity

Clostridium novyi

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Clostridium novyi Toxin A/tcdA (Met1-Glu551).

Target

Toxin A, 3.4.22.-, N-acetylglucosaminyltransferase TcdA, 2.4.1.-, tcdA, toxA

Purification

Purified by antigen affinity column.

Accession

Q46149

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Toxin A (tcdA) is a ~250 kDa protein. Precursor of a cytotoxin, which enters into host cells and mediates autoprocessing to release the active toxin (N-acetylglucosaminyltransferase TcdA) into the host cytosol. Once entered into host cells, acidification in the endosome promotes the membrane insertion of the translocation region and formation of a pore, leading to translocation of the GT44 and peptidase C80 domains across the endosomal membrane. This activates the peptidase C80 domain and autocatalytic processing, releasing the N-terminal part (N-acetylglucosaminyltransferase TcdA), which constitutes the active part of the toxin, in the cytosol.

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1. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

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Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TCDA polyclonal antibody at 1 ug/mL on various samples.
Lane 1: JN055012 100ng

References

Datasheet

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Clostridium novyi Toxin A/tcdA Polyclonal Antibody (JN055014)

Overview

Images

References

Datasheet

SDS

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