Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1) (JN963033)

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Overview

Catalog No.

JN963033

Description

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1) [HS1] (JN963033) is a mouse monoclonal antibody detecting Theta-toxin in ELISA, Neutralization, WB.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Clostridium perfringens (strain 13 / Type A), Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis)

Applications

ELISA, Neutralization, WB

Host species

Mouse

Isotype

IgG2a

Clone ID

HS1

Clonality

Monoclonal

Target

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Streptolysin O, SLO, Thiol-activated cytolysin, slo, Clostridium perfringens (strain 13 / Type A), Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis)

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P0C2E9, Q54114

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Note

For research use only.

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Bioactivity

Detects Clostridium perfringens pfo in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1).
Western blot

Western blot analysis was performed using anti-Theta-toxin monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012)
Lane 2: negative control

References

Datasheet

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Antibody (HS1) (JN963033)

Overview

Images

References

Datasheet

SDS

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