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Anti-CNN3 Polyclonal Antibody (HB438014)

Anti-CNN3 Polyclonal Antibody
Anti-CNN3 Polyclonal Antibody
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Overview
Catalog No.HB438014
Description
Anti-CNN3 Polyclonal Antibody (HB438014) is a rabbit polyclonal antibody detecting acidic isoform; CNN3 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human CNN3 (Met1-Gly133).
Target Calponin-3; Calponin, acidic isoform; CNN3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q15417, Q32L92, P37397, Q9DAW9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Calponin-3 (CNN3) is a ~36 kDa protein. Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Note For research use only
Images
  • Anti-CNN3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CNN3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: NIH3T3 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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