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Anti-Coxiella burnetii ompA Polyclonal Antibody (JN020014)

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Overview
Catalog No.JN020014
Description
Anti-Coxiella burnetii ompA Polyclonal Antibody (JN020014) is a rabbit polyclonal antibody detecting ompA. Suitable for Coxiella burnetii.
Species reactivityCoxiella burnetii
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Coxiella burnetii ompA (Gly1-Gly85).
Target ompA
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession A0A7G6J4B0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Outer membrane protein A (ompA) is a ~37 kDa protein. With TolR probably plays a role in maintaining the position of the peptidoglycan cell wall in the periplasm (Probable). Plays a role in resistance to environmental stress, and a role in outer membrane functionality and cell shape. Non-covalently binds peptidoglycan (Probable). Acts as a porin with low permeability that allows slow penetration of small solutes. A very abundant protein, there can be up to 210,000 OmpA molecules per cell.

1. Wang, Y. (2002) Biochemical and biophysical research communications 292, 396-401. PMID: 11906175
2. Sonntag, I. et al. (1978) Journal of bacteriology 136, 280-5. PMID: 361695
3. Ishida, H. et al. (2014) Biochimica et biophysica acta 1838, 3014-24. PMID: 25135663
4. Sugawara, E. et al. (1992) The Journal of biological chemistry 267, 2507-11. PMID: 1370823
5. Negoda, A. et al. (2010) Biochimica et biophysica acta 1798, 1480-4. PMID: 20004640
6. Negoda, A. et al. (2010) The FEBS journal 277, 4427-37. PMID: 21069910
7. Li, GW. et al. (2014) Cell 157, 624-35. PMID: 24766808
8. Sugawara, E. et al. (1994) The Journal of biological chemistry 269, 17981-7. PMID: 7517935
9. Arora, A. et al. (2000) The Journal of biological chemistry 275, 1594-600. PMID: 10636850
10. Zakharian, E. et al. (2005) Biochemistry 44, 6701-7. PMID: 15850404
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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