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Anti-CPT2 Polyclonal Antibody (HA586014)

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Overview
Catalog No.HA586014
Description
Anti-CPT2 Polyclonal Antibody (HA586014) is a rabbit polyclonal antibody detecting CPT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CPT2 (Thr42-Ser658).
Target Carnitine O-palmitoyltransferase 2 mitochondrial, EC:2.3.1.21, Carnitine palmitoyltransferase II, CPT II, CPT2, CPT1
Endotoxin level Please contact with the lab for this information.
Observed Molecular Weight ~70 kDa
Purification Purified by antigen affinity column.
Accession P23786, P52825, Q2KJB7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) is a ~73 kDa protein. Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites. Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters.

1. Violante, S. et al. (2010) Biochimica et biophysica acta 1802, 728-32. PMID: 20538056
2. Yasuno, T. et al. (2014) Biochemical and biophysical research communications 448, 175-81. PMID: 24780397
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CPT2 Polyclonal Antibody

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