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Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (HF558014)

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Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody
Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody
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Overview
Catalog No.HF558014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CRYAB (Met1-Lys175).
Target Alpha(B)-crystallin, Alpha-crystallin B chain, CRYA2, CRYAB, HSPB5, Heat shock protein beta-5, Heat shock protein family B member 5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P02511
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Alpha-crystallin B chain (CRYAB) is a ~20 kDa protein. May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome.

Note For research use only.
Images
  • Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CRYAB polyclonal antibody at CRYAB on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Rat heart lysate
    Lane 3: Mouse eye lysate
    Lane 4: Rat eye lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody

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