Anti-CRYL1 Polyclonal Antibody (HV315014)

Name: Anti-CRYL1 Polyclonal Antibody
Brand: abinScience
SKU: HV315014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV315014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CRYL1 (Met1-Gln319).

Target

Gul3DH, CRY, L-gulonate 3-dehydrogenase, Lambda-crystallin homolog, CRYL1

Purification

Purified by antigen affinity column.

Accession

Q9Y2S2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lambda-crystallin homolog (CRYL1) is a ~35 kDa protein. Catalyzes the conversion of L-gulonate to 3-dehydro-L-gulonate. It also exhibits low dehydrogenase activity toward L-3-hydroxybutyrate (HBA) and L-threonate. Utilizes NAD as the sole cosubstrate and shows no activity with NADP.

1. Ishikura, S. et al. (2005) Journal of biochemistry 137, 303-14. PMID: 15809331

2. Malatesta, M. et al. (2025) Proceedings of the National Academy of Sciences of the United States of America 122, e2506597122. PMID: 40737316

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CRYL1 (HV315014) at a concentration of 23.75 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CRYL1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-CRYL1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MOuse liver lysate

References

Datasheet

Anti-CRYL1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CRYL1 Polyclonal Antibody (HV315014)

Overview

Images

References

Datasheet

SDS

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