Anti-CRYZ Polyclonal Antibody (HB380014)

Name: Anti-CRYZ Polyclonal Antibody
Brand: abinScience
SKU: HB380014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB380014

Description

Anti-CRYZ Polyclonal Antibody (HB380014) is a rabbit polyclonal antibody detecting Quinone oxidoreductase; 1.6.5.5; NADPH:quinone reductase; Zeta-crystallin; CRYZ in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human CRYZ (Met1-Leu329).

Target

Quinone oxidoreductase; 1.6.5.5; NADPH:quinone reductase; Zeta-crystallin; CRYZ

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q08257, O97764

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Zeta-crystallin (CRYZ) is a ~35 kDa protein. Multifunctional crystallin endowed with unrelated mRNA stabilization and enzymatic oxidoreductase activities. Binds (AU)-rich elements (ARE) in the 3'-UTR of target mRNA to enhance their stability. Upon metabolic acidosis, stabilizes the mRNA encoding SLC12A1 a cotransporter involved in transepithelial NH4(+) reabsorption in the medullary thick ascending limb in kidney. In response to acidosis, also participates to the adaptive increase in renal ammoniagenesis by stabilizing the mRNAs of enzymes involved in glutamine catabolism. By the same mechanism, it also regulates the expression of the antiapoptotic protein BCL2.

1. Fernández, MR. et al. (2007) Cellular and molecular life sciences : CMLS 64, 1419-27. PMID: 17497241

2. Lapucci, A. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 1852-65. PMID: 20103721

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CRYZ polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Raji cell lysate

References

Datasheet

Anti-CRYZ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CRYZ Polyclonal Antibody (HB380014)

Overview

Images

References

Datasheet

SDS