Anti-CSRP3 Polyclonal Antibody (HA661014)

Name: Anti-CSRP3 Polyclonal Antibody
Brand: abinScience
SKU: HA661014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA661014

Description

Anti-CSRP3 Polyclonal Antibody (HA661014) is a rabbit polyclonal antibody detecting cardiac; Muscle LIM protein; CSRP3; CLP; MLP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human CSRP3 (Met1-Glu194).

Target

Cysteine and glycine-rich protein 3; Cardiac LIM protein; Cysteine-rich protein 3; CRP3; LIM domain protein, cardiac; Muscle LIM protein; CSRP3; CLP; MLP

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P50461, P50462, Q4U0T9, P50463

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cysteine and glycine-rich protein 3 (CSRP3) is a ~20 kDa protein. Positive regulator of myogenesis. Acts as a cofactor for myogenic bHLH transcription factors such as MYOD1, and probably MYOG and MYF6. Enhances the DNA-binding activity of the MYOD1:TCF3 isoform E47 complex and may promote formation of a functional MYOD1:TCF3 isoform E47:MEF2A complex involved in myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing.

1. Papalouka, V. et al. (2009) Molecular and cellular biology 29, 6046-58. PMID: 19752190

2. Hoffmann, C. et al. (2014) Molecular and cellular biology 34, 3053-65. PMID: 24934443

3. Lange, S. et al. (2016) Nature communications 7, 12120. PMID: 27353086

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CSRP3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Rat heart lysate
Lane 3: Rat skeletal muscle lysate

References

Datasheet

Anti-CSRP3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CSRP3 Polyclonal Antibody (HA661014)

Overview

Images

References

Datasheet

SDS

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