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Anti-CYP3A7 Polyclonal Antibody (HB872014)

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Overview
Catalog No.HB872014
Description
Anti-CYP3A7 Polyclonal Antibody (HB872014) is a rabbit polyclonal antibody detecting CYP3A7 in ELISA, IHC, WB. Suitable for Human, Callithrix jacchus, and Macaca fascicularis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CYP3A7 (Pro344-Asp497).
Target CYP3A7, CYPIIIA7, P450HLp2, Cytochrome P450 3A7, Cytochrome P450-HFLA
Purification Purified by antigen affinity column.
Accession P24462
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Cytochrome P450 3A7 (CYP3A7) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA), a precursor in the biosynthesis of androgen and estrogen steroid hormones. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1), particularly D-ring hydroxylated estrone at the C16-alpha position.

1. Marill, J. et al. (2000) Molecular pharmacology 58, 1341-8. PMID: 11093772
2. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317
3. Lee, AJ. et al. (2003) Cancer research 63, 6532-6. PMID: 14559847
4. Torimoto, N. et al. (2007) Drug metabolism and disposition: the biological fate of chemicals 35, 484-92. PMID: 17178770
5. Ohmori, S. et al. (1998) Biochimica et biophysica acta 1380, 297-304. PMID: 9555064
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-CYP3A7 Polyclonal Antibody

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