Anti-DAAM1 Polyclonal Antibody (HV298014)

Name: Anti-DAAM1 Polyclonal Antibody
Brand: abinScience
SKU: HV298014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV298014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human DAAM1 (Met1-Tyr250).

Target

KIAA0666, DAAM1, Disheveled-associated activator of morphogenesis 1

Purification

Purified by antigen affinity column.

Accession

Q9Y4D1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Disheveled-associated activator of morphogenesis 1 (DAAM1) is a ~123 kDa protein. Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells.

1. Aspenström, P. et al. (2006) Experimental cell research 312, 2180-94. PMID: 16630611

2. Lu, J. et al. (2007) Journal of molecular biology 369, 1258-69. PMID: 17482208

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DAAM1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse liver lysate
Lane 5: Mouse brain lysate
Lane 6: Mouse heart lysate

References

Datasheet

Anti-DAAM1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DAAM1 Polyclonal Antibody (HV298014)

Overview

Images

References

Datasheet

SDS

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