Anti-DAZAP2 Polyclonal Antibody (HB402014)

Name: Anti-DAZAP2 Polyclonal Antibody
Brand: abinScience
SKU: HB402014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB402014

Description

Anti-DAZAP2 Polyclonal Antibody (HB402014) is a rabbit polyclonal antibody detecting DAZ-associated protein 2; Deleted in azoospermia-associated protein 2; Proline-rich transcript in brain protein; DAZAP2; KIAA0058; PRTB in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human DAZAP2 (Ala41-Trp168).

Target

DAZ-associated protein 2; Deleted in azoospermia-associated protein 2; Proline-rich transcript in brain protein; DAZAP2; KIAA0058; PRTB

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q15038, Q3T0K9, Q9DCP9, P60486

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DAZ-associated protein 2 (DAZAP2) is a ~17 kDa protein. In unstressed cells, promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters. This limits the expression of a number of cell death-mediating TP53 target genes, reducing DNA damage-induced cell death. Enhances the binding of transcription factor TCF7L2/TCF4, a Wnt signaling pathway effector, to the promoters of target genes. Plays a role in stress granule formation.

1. Liebl, MC. et al. (2021) Nucleic acids research 49, 2759-2776. PMID: 33591310

2. Kim, JE. et al. (2008) Molecular and cellular biology 28, 803-13. PMID: 17984221

Note

For research use only

Images

References

Datasheet

Anti-DAZAP2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DAZAP2 Polyclonal Antibody (HB402014)

Overview

Images

References

Datasheet

SDS