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Anti-DCAF15 Polyclonal Antibody (HB522014)

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Overview
Catalog No.HB522014
Description
Anti-DCAF15 Polyclonal Antibody (HB522014) is a rabbit polyclonal antibody detecting DDB1- and CUL4-associated factor 15; DCAF15; C19orf72 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human DCAF15 (Tyr386-Val564).
Target DDB1- and CUL4-associated factor 15; DCAF15; C19orf72
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q66K64, Q3SZD5, Q6PFH3, A2BDA5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DDB1- and CUL4-associated factor 15 (DCAF15) is a ~66 kDa protein. Substrate-recognition component of the DCX(DCAF15) complex, a cullin-4-RING E3 ubiquitin-protein ligase complex that mediates ubiquitination and degradation of target proteins. The DCX(DCAF15) complex acts as a regulator of the natural killer (NK) cells effector functions, possibly by mediating ubiquitination and degradation of cohesin subunits SMC1A and SMC3. May play a role in the activation of antigen-presenting cells (APC) and their interaction with NK cells.

1. Jin, J. et al. (2006) Molecular cell 23, 709-21. PMID: 16949367
2. Pech, MF. et al. (2019) eLife 8. PMID: 31452512
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-DCAF15 Polyclonal Antibody

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