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Anti-DCD Polyclonal Antibody (HX130014)

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Overview
Catalog No.HX130014
Description
Anti-DCD Polyclonal Antibody (HX130014) is a rabbit polyclonal antibody detecting DCD in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DCD (Cys18-Leu110).
Target DCD, AIDD, DSEP, Dermcidin, Preproteolysin
Purification Purified by antigen affinity column.
Accession P81605
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Dermcidin (DCD) is a ~11 kDa protein. Found in sweat, has an antimicrobial activity during early bacterial colonization. The secreted peptide assembles into homohexameric complexes that can associate with and also insert into pathogen membranes. Once inserted in bacteria membranes forms anion channels probably altering the transmembrane potential essential for bacterial survival. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat.

1. Schittek, B. et al. (2001) Nature immunology 2, 1133-7. PMID: 11694882
2. Song, C. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 4586-91. PMID: 23426625
3. Lee Motoyama, JP. et al. (2007) Biochemical and biophysical research communications 357, 828-33. PMID: 17448443
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DCD Polyclonal Antibody

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