Anti-DDB1 Polyclonal Antibody (HS873014)

Name: Anti-DDB1 Polyclonal Antibody
Brand: abinScience
SKU: HS873014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
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Overview

Catalog No.

HS873014

Description

Anti-DDB1 Polyclonal Antibody (HS873014) is a rabbit polyclonal antibody detecting DNA damage-binding protein 1; DDB p127 subunit; DNA damage-binding protein a; DDBa; Damage-specific DNA-binding protein 1; HBV X-associated protein 1; XAP-1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XPE-binding factor; XPE-BF; Xeroderma pigmentosum group E-complementing protein; XPCe; DDB1; XAP1 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, Rat, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human DDB1 (Gly393-Gln708).

Target

DNA damage-binding protein 1; DDB p127 subunit; DNA damage-binding protein a; DDBa; Damage-specific DNA-binding protein 1; HBV X-associated protein 1; XAP-1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XPE-binding factor; XPE-BF; Xeroderma pigmentosum group E-complementing protein; XPCe; DDB1; XAP1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q16531, A1A4K3, Q3U1J4, Q9ESW0, Q805F9, Q6P6Z0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA damage-binding protein 1 (DDB1) is a ~126 kDa protein. Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1.

1. Wertz, IE. et al. (2004) Science (New York, N.Y.) 303, 1371-4. PMID: 14739464

2. Hu, J. et al. (2004) Nature cell biology 6, 1003-9. PMID: 15448697

3. Kulaksiz, G. et al. (2005) Molecular and cellular biology 25, 9784-92. PMID: 16260596

4. Hu, J. et al. (2006) The Journal of biological chemistry 281, 3753-6. PMID: 16407242

5. Senga, T. et al. (2006) The Journal of biological chemistry 281, 6246-52. PMID: 16407252

6. Nishitani, H. et al. (2006) The EMBO journal 25, 1126-36. PMID: 16482215

7. Lovejoy, CA. et al. (2006) Molecular and cellular biology 26, 7977-90. PMID: 16940174

8. He, YJ. et al. (2006) Genes & development 20, 2949-54. PMID: 17079684

9. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626

Note

For research use only

Images

References

Datasheet

Anti-DDB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-DDB1 Polyclonal Antibody (HS873014)

Overview

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Datasheet

SDS

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