Anti-DDX4 Polyclonal Antibody (HV699014)

Name: Anti-DDX4 Polyclonal Antibody
Brand: abinScience
SKU: HV699014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV699014

Description

Anti-DDX4 Polyclonal Antibody (HV699014) is a rabbit polyclonal antibody detecting DDX4 in ELISA, IHC, WB. Suitable for Human, Bovine, Macaca fascicularis, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DDX4 (Asn35-Gly131).

Target

DDX4, VASA, Vasa homolog, DEAD box protein 4, Probable ATP-dependent RNA helicase DDX4

Purification

Purified by antigen affinity column.

Accession

Q9NQI0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Probable ATP-dependent RNA helicase DDX4 (Vasa) is a ~79 kDa protein. ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.

1. Castrillon, DH. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 9585-90. PMID: 10920202

2. Li, HJ. et al. (2010) Chinese medical journal 123, 2879-82. PMID: 21034600

Note

For research use only.

Images

References

Datasheet

Anti-DDX4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DDX4 Polyclonal Antibody (HV699014)

Overview

Images

References

Datasheet

SDS

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