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Anti-DDX6 Polyclonal Antibody (HA609014)

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Overview
Catalog No.HA609014
Description
Anti-DDX6 Polyclonal Antibody (HA609014) is a rabbit polyclonal antibody detecting Probable ATP-dependent RNA helicase DDX6; 3.6.4.13; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK; DDX6; HLR2; RCK in ELISA, IHC, WB. Suitable for Human, Chicken, Mouse, and Frog.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human DDX6 (Glu97-Pro483).
Target Probable ATP-dependent RNA helicase DDX6; 3.6.4.13; ATP-dependent RNA helicase p54; DEAD box protein 6; Oncogene RCK; DDX6; HLR2; RCK
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P26196, Q5ZKB9, P54823, P54824
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Probable ATP-dependent RNA helicase DDX6 is a ~54 kDa protein. Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.

1. Ayache, J. et al. (2015) Molecular biology of the cell 26, 2579-95. PMID: 25995375
2. Kamenska, A. et al. (2016) Nucleic acids research 44, 6318-34. PMID: 27342281
3. Balak, C. et al. (2019) American journal of human genetics 105, 509-525. PMID: 31422817
4. Fenger-Grøn, M. et al. (2005) Molecular cell 20, 905-15. PMID: 16364915
5. Hu, G. et al. (2015) Nature cell biology 17, 930-942. PMID: 26098573
Note For research use only
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Datasheet
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Formula
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Anti-DDX6 Polyclonal Antibody

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