Anti-DEFA3 Polyclonal Antibody (HX987014)

Name: Anti-DEFA3 Polyclonal Antibody
Brand: abinScience
SKU: HX987014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX987014

Description

Anti-DEFA3 Polyclonal Antibody (HX987014) is a rabbit polyclonal antibody detecting DEFA3 in ELISA, IHC, WB. Suitable for Human, Macaca mulatta, Pan troglodytes, and Papio hamadryas.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Macaca mulatta, Pan troglodytes, Papio hamadryas

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DEFA3 (Pro21-Cys94).

Target

HP3, Neutrophil defensin 3, HNP-3, HNP-2, DEF3, HP-3, Defensin, alpha 3, DEFA3, HP-2, HP2

Purification

Purified by antigen affinity column.

Accession

P59666

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Neutrophil defensin 3 (DEFA3) is a ~10 kDa protein. Effector molecule of the innate immune system that acts via antibiotic-like properties against a broad array of infectious agents including bacteria, fungi, and viruses. Possesses the ability to neutralize bacterial toxins such as B.anthracis lethal factor, Clostridium difficile cytotoxin B as well as leukocidin produced by Staphylococcus aureus. Also blocks herpes simplex virus infection by interacting with envelope glycoprotein B and thus preventing its binding to heparan sulfate, the receptor for attachment.

1. Ericksen, B. et al. (2005) Antimicrobial agents and chemotherapy 49, 269-75. PMID: 15616305

2. Kim, C. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 4830-5. PMID: 15772169

3. Hazrati, E. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 177, 8658-66. PMID: 17142766

4. Giesemann, T. et al. (2008) Gastroenterology 134, 2049-58. PMID: 18435932

5. Cardot-Martin, E. et al. (2015) Letters in applied microbiology 61, 158-64. PMID: 25963798

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DEFA3 polyclonal antibody at 1ug/mL on recombinant DEFA3 (Catalog No. HX987012).

References

Datasheet

Anti-DEFA3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-DEFA3 Polyclonal Antibody (HX987014)

Overview

Images

References

Datasheet

SDS

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