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Anti-DENV-3 Envelope protein E Polyclonal Antibody (VK589014)

Anti-DENV-3 Envelope protein E Polyclonal Antibody
Anti-DENV-3 Envelope protein E Polyclonal Antibody
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Overview
Catalog No.VK589014
Description
Anti-DENV-3 Envelope protein E Polyclonal Antibody (VK589014) is a rabbit polyclonal antibody detecting Envelope protein E. Suitable for Dengue virus type 3.
Species reactivityDengue virus 3 (DENV-3)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant DENV-3 Envelope protein E (Met575-Lys678).
Target Capsid protein C, Core protein, EC:2.1.1.56, EC:2.1.1.57, EC:2.7.7.48, EC:3.4.21.91, EC:3.6.1.15, EC:3.6.4.13, Envelope protein E, Flavivirin protease NS2B regulatory subunit, Flavivirin protease NS3 catalytic subunit, Genome polyprotein, Matrix protein, NS1, NS2A, NS4A, NS4B, Non-structural protein 1, Non-structural protein 2A, Non-structural protein 2B, Non-structural protein 3, Non-structural protein 4A, Non-structural protein 4B, Non-structural protein 5, Peptide 2k, Peptide pr, Protein prM, RNA-directed RNA polymerase/Methyltransferase NS5, Serine protease NS3, Serine protease subunit NS2B, Small envelope protein M
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P27915
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Genome polyprotein (Envelope protein E) is a ~378 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.

Note For research use only
Images
  • Anti-DENV-3 Envelope protein E Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-P3-EC polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant DENV-3 Envelope protein E (Catalog No: VK589022)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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