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Anti-DGKQ Polyclonal Antibody (HW435014)

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Overview
Catalog No.HW435014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DGKQ (Pro584-Ala721).
Target DGKQ, Diglyceride kinase theta, DGK-theta, DAG kinase theta, DGKtheta, DAGK4, Diacylglycerol kinase theta
Purification Purified by antigen affinity column.
Accession P52824
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Diacylglycerol kinase theta (DGKQ) is a ~101 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription. Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor. Through its diacylglycerol activity also regulates synaptic vesicle endocytosis.

1. Bregoli, L. et al. (2001) The Journal of biological chemistry 276, 23288-95. PMID: 11309392
2. Gellett, AM. et al. (2012) Biochemical and biophysical research communications 422, 758-63. PMID: 22627129
3. Houssa, B. et al. (1997) The Journal of biological chemistry 272, 10422-8. PMID: 9099683
4. Li, D. et al. (2007) Molecular and cellular biology 27, 6669-85. PMID: 17664281
5. Goldschmidt, HL. et al. (2016) Cell reports 14, 200-7. PMID: 26748701
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-DGKQ Polyclonal Antibody

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