Anti-DHFR Polyclonal Antibody (HF957014)

Name: Anti-DHFR Polyclonal Antibody
Brand: abinScience
SKU: HF957014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HF957014

Description

Anti-DHFR Polyclonal Antibody (HF957014) is a rabbit polyclonal antibody detecting DHFR in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DHFR (Met1-Asp187).

Target

Dihydrofolate reductase, DHFR

Purification

Purified by antigen affinity column.

Accession

P00374

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dihydrofolate reductase (DHFR) is a ~21 kDa protein. Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

1. Klon, AE. et al. (2002) Journal of molecular biology 320, 677-93. PMID: 12096917

2. Cody, V. et al. (2004) Acta crystallographica. Section D, Biological crystallography 60, 646-55. PMID: 15039552

3. Reynolds, RC. et al. (2007) Journal of medicinal chemistry 50, 3283-9. PMID: 17569517

4. Cody, V. et al. (2009) Biochemistry 48, 1702-11. PMID: 19196009

5. Volpato, JP. et al. (2009) The Journal of biological chemistry 284, 20079-89. PMID: 19478082

6. McEntee, G. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15157-62. PMID: 21876184

7. Gangjee, A. et al. (1998) Journal of medicinal chemistry 41, 3426-34. PMID: 9719595

8. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188

9. Anderson, DD. et al. (2012) The Journal of biological chemistry 287, 7051-62. PMID: 22235121

Note

For research use only.

Images

References

Datasheet

Anti-DHFR Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DHFR Polyclonal Antibody (HF957014)

Overview

Images

References

Datasheet

SDS

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