

| Catalog No. | HT055014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Description |
Anti-DIAPH1 Polyclonal Antibody (HT055014) is a rabbit polyclonal antibody detecting DIAPH1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
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| Species reactivity | Human, Mouse | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Host species | Rabbit | ||||||||
| Isotype | IgG | ||||||||
| Clonality | Polyclonal | ||||||||
| Immunogen | E. coli - derived recombinant Human DIAPH1 (Asp389-Pro583). | ||||||||
| Target | Protein diaphanous homolog 1, DIAP1, DRF1, Diaphanous-related formin-1, DIAPH1 | ||||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Accession | O60610 | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Background | Protein diaphanous homolog 1 (DIAPH1) is a ~141 kDa protein. Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. 1. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854 2. Bai, SW. et al. (2011) BMC biology 9, 54. PMID: 21834987 3. Stritt, S. et al. (2016) Blood 127, 2903-14. PMID: 26912466 | ||||||||
| Note | For research use only. |
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