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Anti-DIO1 Polyclonal Antibody (HW476014)

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Overview
Catalog No.HW476014
Description
Anti-DIO1 Polyclonal Antibody (HW476014) is a rabbit polyclonal antibody detecting DIO1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DIO1 (Gly89-Ser254).
Target Type I iodothyronine deiodinase, 1.21.99.4, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1
Purification Purified by antigen affinity column.
Accession P49895
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Type I iodothyronine deiodinase (DIO1) is a ~28 kDa protein. Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (3,3'-T2) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD). Catalyzes the deiodination of T4 to 3,3',5'-triiodothyronine (rT3) via inner-ring deiodination (IRD). Catalyzes the deiodination of T3 to 3,3'-T2, 3,5-diiodothyronine (3,5-T2) to 3- monoiodothyronine (3-T1) and 3,3'-T2 to 3-T1 via IRD. Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine and 3',5'-diiodothyronamine.

1. Curcio-Morelli, C. et al. (2003) Endocrinology 144, 937-46. PMID: 12586771
2. França, MM. et al. (2021) Thyroid : official journal of the American Thyroid Association 31, 202-207. PMID: 32718224
3. Piehl, S. et al. (2008) Rapid communications in mass spectrometry : RCM 22, 3286-96. PMID: 18821722
4. Piehl, S. et al. (2008) Endocrinology 149, 3037-45. PMID: 18339710
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-DIO1 Polyclonal Antibody

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