Anti-DLAT Polyclonal Antibody (HY587014)

Name: Anti-DLAT Polyclonal Antibody
Brand: abinScience
SKU: HY587014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY587014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DLAT (Gln374-Trp631).

Target

DLTA, M2 antigen complex 70 kDa subunit, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Pyruvate dehydrogenase complex component E2, PDC-E2, DLAT, PBC, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, PDCE2

Purification

Purified by antigen affinity column.

Accession

P10515

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT/PDC-E2) is a ~68 kDa protein. The pyruvate dehydrogenase (PDH) complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3); (Probable). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acetyl groups from acetyl-lipoyl moiety generated by the pyruvate dehydrogenase, leading to acetyl-CoA formation (Probable).

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DLAT polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Lncap cell lysate
Lane 4: K562 cell lysate
Lane 5: HCT116 cell lysate
Lane 6: NIH3T3 cell lysate
Lane 7: Mouse brain lysate
Lane 8: Mouse liver lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate
Lane11: Rat liver lysate
Lane12: Rat kidney lysate