Anti-DLD Polyclonal Antibody (HY347014)

Name: Anti-DLD Polyclonal Antibody
Brand: abinScience
SKU: HY347014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY347014

Description

Anti-DLD Polyclonal Antibody (HY347014) is a rabbit polyclonal antibody detecting DLD in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DLD (Lys283-His487).

Target

Glycine cleavage system L protein, Dihydrolipoamide dehydrogenase, GCSL, DLD, PHE3, Dihydrolipoyl dehydrogenase, mitochondrial, LAD

Purification

Purified by antigen affinity column.

Accession

P09622

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dihydrolipoyl dehydrogenase, mitochondrial (DLD) is a ~54 kDa protein. Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

1. Odièvre, MH. et al. (2005) Human mutation 25, 323-4. PMID: 15712224

2. Brautigam, CA. et al. (2006) Structure (London, England : 1993) 14, 611-21. PMID: 16442803

3. Cameron, JM. et al. (2006) American journal of medical genetics. Part A 140, 1542-52. PMID: 16770810

4. Babady, NE. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6158-63. PMID: 17404228

5. Patel, MS. et al. (2009) Journal of molecular catalysis. B, Enzymatic 61, 2-6. PMID: 20160912

6. Park, YH. et al. (2010) Biochemical and biophysical research communications 395, 416-9. PMID: 20385101

7. Wang, Y. et al. (2017) Nature 552, 273-277. PMID: 29211711

Note

For research use only.

Images

References

Datasheet

Anti-DLD Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DLD Polyclonal Antibody (HY347014)

Overview

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References

Datasheet

SDS

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