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Anti-DLG1 Polyclonal Antibody (HC695014)

Anti-DLG1 Polyclonal Antibody
Anti-DLG1 Polyclonal Antibody
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Overview
Catalog No.HC695014
Description
Anti-DLG1 Polyclonal Antibody (HC695014) is a rabbit polyclonal antibody detecting Disks large homolog 1; Synapse-associated protein 97; SAP-97; SAP97; hDlg; DLG1 in ELISA, IHC, WB. Suitable for Human, Dog, Mouse, Rat, and Zebrafish.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human DLG1 (Asn214-Met410).
Target Disks large homolog 1; Synapse-associated protein 97; SAP-97; SAP97; hDlg; DLG1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q12959, A0A8C0TYJ0, Q811D0, Q62696, Q5PYH5, Q5PYH6, Q62108, P31016, P78352
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Disks large homolog 1 (DLG1) is a ~100 kDa protein. Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion. May also play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels.

1. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497
Note For research use only
Images
  • Anti-DLG1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-DLG1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: U87 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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