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Anti-DNAJA1 Polyclonal Antibody (HB223014)

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Overview
Catalog No.HB223014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DNAJA1 (Asn106-Asn327).
Target DnaJ protein homolog 2, DNAJA1, Heat shock protein J2, HDJ2, HSJ-2, HSJ2, HSPF4, Heat shock 40 kDa protein 4, HSDJ, DNAJ2, DnaJ homolog subfamily A member 1, Human DnaJ protein 2, hDj-2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P31689
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DnaJ homolog subfamily A member 1 (DNAJA1) is a ~44 kDa protein. Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.

1. Terada, K. et al. (2000) The Journal of biological chemistry 275, 24728-34. PMID: 10816573
2. Rauch, JN. et al. (2014) The Journal of biological chemistry 289, 1402-14. PMID: 24318877
3. Gotoh, T. et al. (2004) Cell death and differentiation 11, 390-402. PMID: 14752510
4. Stark, JL. et al. (2014) Biochemistry 53, 1360-72. PMID: 24512202
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DNAJA1 Polyclonal Antibody

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