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Anti-DOCK4 Polyclonal Antibody (HV518014)

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Overview
Catalog No.HV518014
Description
Anti-DOCK4 Polyclonal Antibody (HV518014) is a rabbit polyclonal antibody detecting DOCK4 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DOCK4 (Met1-Pro300).
Target DOCK4, KIAA0716, Dedicator of cytokinesis protein 4
Purification Purified by antigen affinity column.
Accession Q8N1I0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Dedicator of cytokinesis protein 4 (DOCK4) is a ~225 kDa protein. Functions as a guanine nucleotide exchange factor (GEF) that promotes the exchange of GDP to GTP, converting inactive GDP-bound small GTPases into their active GTP-bound form. Involved in regulation of adherens junction between cells. Plays a role in cell migration.

1. Yajnik, V. et al. (2003) Cell 112, 673-84. PMID: 12628187
2. Yan, D. et al. (2006) Journal of molecular biology 357, 755-64. PMID: 16464467
3. Hiramoto-Yamaki, N. et al. (2010) The Journal of cell biology 190, 461-77. PMID: 20679435
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-DOCK4 Polyclonal Antibody

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