Anti-DPP8 Polyclonal Antibody (HP914014)

Name: Anti-DPP8 Polyclonal Antibody
Brand: abinScience
SKU: HP914014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HP914014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human DPP8 (Met8-Gln199).

Target

DPP VIII, DPRP-1, Dipeptidyl peptidase 8, Dipeptidyl peptidase VIII, DPRP1, DP8, DPP8, Dipeptidyl peptidase IV-related protein 1, Prolyl dipeptidase DPP8

Purification

Purified by antigen affinity column.

Accession

Q6V1X1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Dipeptidyl peptidase 8 (DPP8) is a ~103 kDa protein. Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8. Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation. The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition.

1. Abbott, CA. et al. (2000) European journal of biochemistry 267, 6140-50. PMID: 11012666

2. Ajami, K. et al. (2003) Biochemistry 42, 694-701. PMID: 12534281

3. Qi, SY. et al. (2003) The Biochemical journal 373, 179-89. PMID: 12662155

4. Chen, YS. et al. (2004) Protein expression and purification 35, 142-6. PMID: 15039077

5. Jiaang, WT. et al. (2005) Bioorganic & medicinal chemistry letters 15, 687-91. PMID: 15664838

6. Pitman, MR. et al. (2010) Biological chemistry 391, 959-72. PMID: 20536396

7. Ross, B. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1437-E1445. PMID: 29382749

8. Okondo, MC. et al. (2017) Nature chemical biology 13, 46-53. PMID: 27820798

9. Johnson, DC. et al. (2018) Nature medicine 24, 1151-1156. PMID: 29967349

10. Johnson, DC. et al. (2020) Cell death & disease 11, 628. PMID: 32796818

Note

For research use only.

Images

References

Datasheet

Anti-DPP8 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-DPP8 Polyclonal Antibody (HP914014)

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Datasheet

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