Anti-DUSP11 Polyclonal Antibody (HF776014)

Name: Anti-DUSP11 Polyclonal Antibody
Brand: abinScience
SKU: HF776014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF776014

Description

Anti-DUSP11 Polyclonal Antibody (HF776014) is a rabbit polyclonal antibody detecting DUSP11 in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DUSP11 (Gly75-Trp255).

Target

Phosphatase that interacts with RNA/RNP complex 1, Dual specificity protein phosphatase 11, DUSP11, RNA/RNP complex-1-interacting phosphatase, PIR1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O75319

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RNA/RNP complex-1-interacting phosphatase (DUSP11) is a ~38 kDa protein. Possesses RNA 5'-triphosphatase and diphosphatase activities, but displays a poor protein-tyrosine phosphatase activity. In addition, has phosphatase activity with ATP, ADP and O-methylfluorescein phosphate (in vitro). Binds to RNA. May participate in nuclear mRNA metabolism.

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting DUSP11 (HF776014) at a concentration of 38.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of DUSP11 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet

Anti-DUSP11 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-DUSP11 Polyclonal Antibody (HF776014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us