Anti-E. coli groEL Polyclonal Antibody (JN145014)

Name: Anti-E. coli groEL Polyclonal Antibody
Brand: abinScience
SKU: JN145014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

JN145014

Description

Anti-E. coli groEL Polyclonal Antibody (JN145014) is a rabbit polyclonal antibody detecting GroEL protein. Suitable for strain K12.

Species reactivity

Escherichia coli

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant E. coli groEL (Met1-Met548).

Target

60 kDa chaperonin, Chaperonin GroEL, Chaperonin-60, Cpn60, EC:5.6.1.7, GroEL protein, groEL, groL, mopA

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P0A6F5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Chaperonin GroEL is a ~57 kDa protein. Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. Rapid binding of ATP, followed by slower binding of the non-native substrate protein and GroES to the cis open ring of GroEL initiates productive folding of the non-native protein inside a highly stable GroEL-ATP-GroES complex. Binding of ATP and GroES induces conformational changes that result in the release of the substrate protein into a nano-cage compartment, within the GroEL central cavity, for folding in isolation. To discharge GroES and substrate protein, ATP hydrolysis in the cis ring is required to form a GroEL-ADP-GroES complex with decreased stability.

1. Goloubinoff, P. et al. (1989) Nature 342, 884-9. PMID: 10532860

2. Tang, YC. et al. (2006) Cell 125, 903-14. PMID: 16751100

3. Martin, J. et al. (1991) Nature 352, 36-42. PMID: 1676490

4. Tang, YC. et al. (2008) The EMBO journal 27, 1458-68. PMID: 18418386

5. Apetri, AC. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 17351-5. PMID: 18987317

6. Chakraborty, K. et al. (2010) Cell 142, 112-22. PMID: 20603018

7. Gupta, AJ. et al. (2014) Journal of molecular biology 426, 2739-54. PMID: 24816391

8. Kusukawa, N. et al. (1989) The EMBO journal 8, 3517-21. PMID: 2573517

9. Hemmingsen, SM. et al. (1988) Nature 333, 330-4. PMID: 2897629

10. Horwich, AL. et al. (1993) Cell 74, 909-17. PMID: 8104102

Note

For research use only

Images

References

Datasheet

Anti-E. coli groEL Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-E. coli groEL Polyclonal Antibody (JN145014)

Overview

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References

Datasheet

SDS

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