Anti-EBF1 Polyclonal Antibody (HV735014)

Name: Anti-EBF1 Polyclonal Antibody
Brand: abinScience
SKU: HV735014
Price: 168 USD
Availability: InStock

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Overview
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SDS

Overview

Catalog No.

HV735014

Description

Anti-EBF1 Polyclonal Antibody (HV735014) is a rabbit polyclonal antibody detecting EBF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EBF1 (Asp179-Ser451).

Target

EBF, EBF1, OE-1, COE1, Early B-cell factor, Transcription factor COE1, O/E-1

Purification

Purified by antigen affinity column.

Accession

Q9UH73

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor COE1 (EBF1) is a ~64 kDa protein. Key pioneer transcription factor of B-cell specification and commitment. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold.

1. Yang, CY. et al. (2016) Genes & development 30, 2310-2324. PMID: 27807034

Note

For research use only.

Images

References

Datasheet

Anti-EBF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EBF1 Polyclonal Antibody (HV735014)

Overview

Images

References

Datasheet

SDS

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