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Anti-E.coli lexA/LexA repressor Recombinant Nanobody (SAA1458) (JN826013)

Anti-E.coli lexA/LexA repressor Recombinant Nanobody (SAA1458)
Anti-E.coli lexA/LexA repressor Recombinant Nanobody (SAA1458)
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Overview
Catalog No.JN826013
Description
Anti-E.coli lexA/LexA repressor Nanobody (SAA1458) [SAA1458] (JN826013) is a alpaca monoclonal antibody detecting LexA repressor in ELISA.
Species reactivityEscherichia coli
ApplicationsELISA
Host speciesAlpaca
IsotypeVHH-8His-Cys-tag
Clone IDSAA1458
Clonality Monoclonal
Target LexA repressor, lexA
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Accession Q1R3P3
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

LexA repressor is a ~22 kDa protein. Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.

Note For research use only.
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  • Anti-E.coli lexA/LexA repressor Recombinant Nanobody (SAA1458)

    SDS-PAGE

    SDS-PAGE for Anti-E.coli lexA/LexA repressor Nanobody (SAA1458)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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