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Anti-Eculizumab Polyclonal Antibody (AF687014)

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Overview
Catalog No.AF687014
Description
Anti-Eculizumab Polyclonal Antibody (AF687014) is a rabbit polyclonal antibody detecting Eculizumab in ELISA.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityGeneral
ApplicationsELISA
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen Eculizumab
Target Rabbit polyclonal to Soliris.
Specificity The product is specific for Eculizumab. This antibody serves as an excellent positive control for Eculizumab immunogenicity (ADA) assays.
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession CAS: 219685-50-4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
BackgroundEculizumab with trade name Soliris, is an FDA-approved drug to treat paroxysmal nocturnal hemoglobinuria (PNH), atypical hemolytic uremic syndrome (aHUS), and neuromyelitis optica. Eculizumab is a recombinant humanized monoclonal antibody against the complement protein C5. Eculizumab specifically binds to the terminal complement component 5, or C5. Binding to this protein prevents the activation of a complement terminal complex, which is used to treat a number of autoimmune conditions.
• Induction Immunosuppression in High-risk Kidney Transplant Recipients., PMID:27041548
• Acute Rejection Following Kidney Transplantation: State-of-the-Art and Future Perspectives., PMID:32520680
• The impact of terminal complement blockade on the efficacy of induction with polyclonal rabbit antithymocyte globulin in living donor renal allografts., PMID:22813834
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Eculizumab Polyclonal Antibody

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