Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014)

Name: Anti-EDN1/Endothelin1 Polyclonal Antibody
Brand: abinScience
SKU: HY182014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY182014

Description

Anti-EDN1/Endothelin1 Polyclonal Antibody (HY182014) is a rabbit polyclonal antibody detecting Endothelin1 in ELISA, IHC, WB. Suitable for Human and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EDN1 (Ala18-Trp212).

Target

Endothelin-1, EDN1, PPET1, ET-1, Preproendothelin-1, Endothelin1

Purification

Purified by antigen affinity column.

Accession

P05305

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Endothelin-1 (EDN1) is a ~24 kDa protein. Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments. NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2.

1. Rufanova, VA. et al. (2009) Journal of cellular physiology 219, 45-56. PMID: 19086031

2. Glorioso, N. et al. (2007) Circulation research 100, 1522-9. PMID: 17446437

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EDN1/Endothelin1 polyclonal antibody at 1ug/mL on recombinant EDN1/Endothelin1 (Catalog No. HY182012).

References

Datasheet