Anti-EGLN3 Polyclonal Antibody (HP404014)

Name: Anti-EGLN3 Polyclonal Antibody
Brand: abinScience
SKU: HP404014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP404014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human EGLN3 (Met1-Asp239).

Target

PHD3, Hypoxia-inducible factor prolyl hydroxylase 3, Prolyl hydroxylase EGLN3, HPH-3, Prolyl hydroxylase domain-containing protein 3, Egl nine homolog 3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-1, EGLN3

Purification

Purified by antigen affinity column.

Accession

Q9H6Z9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Prolyl hydroxylase EGLN3 is a ~27 kDa protein. Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4, GPX4 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A.

1. Xie, L. et al. (2009) Science signaling 2, ra33. PMID: 19584355

2. Su, Y. et al. (2010) British journal of cancer 103, 1571-9. PMID: 20978507

3. Chen, N. et al. (2011) Cell research 21, 983-6. PMID: 21483450

4. Yan, B. et al. (2011) Biochemical and biophysical research communications 409, 315-20. PMID: 21575608

5. Luo, W. et al. (2011) Cell 145, 732-44. PMID: 21620138

6. Xie, L. et al. (2012) The Journal of clinical investigation 122, 2827-36. PMID: 22797300

7. Zheng, P. et al. (2025) Nature chemical biology 21, 1420-1432. PMID: 40281343

8. Epstein, AC. et al. (2001) Cell 107, 43-54. PMID: 11595184

9. Huang, J. et al. (2002) The Journal of biological chemistry 277, 39792-800. PMID: 12181324

Note

For research use only.

Images

References

Datasheet

Anti-EGLN3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-EGLN3 Polyclonal Antibody (HP404014)

Overview

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Datasheet

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