Anti-EIF2S3 Polyclonal Antibody (HA632014)

Name: Anti-EIF2S3 Polyclonal Antibody
Brand: abinScience
SKU: HA632014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HA632014

Description

Anti-EIF2S3 Polyclonal Antibody (HA632014) is a rabbit polyclonal antibody detecting Eukaryotic translation initiation factor 2 subunit 3; 3.6.5.3; Eukaryotic translation initiation factor 2 subunit gamma X; eIF2-gamma X; eIF2gX; EIF2S3; EIF2G in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, Pig, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human EIF2S3 (Met1-Asp472).

Target

Eukaryotic translation initiation factor 2 subunit 3; 3.6.5.3; Eukaryotic translation initiation factor 2 subunit gamma X; eIF2-gamma X; eIF2gX; EIF2S3; EIF2G

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P41091, Q2VIR3, P81795, Q9Z0N1, P20461, Q2KHU8, Q5ZMS3, Q9Z0N2, C9WPN6, F1QGW6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Eukaryotic translation initiation factor 2 subunit 3 (EIF2S3) is a ~51 kDa protein. Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

1. Young-Baird, SK. et al. (2020) Molecular cell 77, 875-886.e7. PMID: 31836389

Note

For research use only

Images

References

Datasheet

Anti-EIF2S3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-EIF2S3 Polyclonal Antibody (HA632014)

Overview

Images

References

Datasheet

SDS

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