Anti-ENO3 Polyclonal Antibody (HB156014)

Name: Anti-ENO3 Polyclonal Antibody
Brand: abinScience
SKU: HB156014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB156014

Description

Anti-ENO3 Polyclonal Antibody (HB156014) is a rabbit polyclonal antibody detecting ENO3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Salmo salar.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ENO3 (Met1-Gly391).

Target

2-phospho-D-glycerate hydro-lyase, Muscle-specific enolase, Skeletal muscle enolase, ENO3, Enolase 3, Beta-enolase, MSE

Purification

Purified by antigen affinity column.

Accession

P13929

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Beta-enolase (ENO3) is a ~46 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Appears to have a function in striated muscle development and regeneration.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ENO3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse heart lysate
Lane 8: Mouse skeletal muscle lysate
Lane 9: Rat brain lysate
Lane 10: Rat heart lysate
Lane11: Rat skeletal muscle lysate

References

Datasheet

Anti-ENO3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ENO3 Polyclonal Antibody (HB156014)

Overview

Images

References

Datasheet

SDS

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