Anti-EPOR Polyclonal Antibody (HB152014)

Name: Anti-EPOR Polyclonal Antibody
Brand: abinScience
SKU: HB152014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB152014

Description

Anti-EPOR Polyclonal Antibody (HB152014) is a rabbit polyclonal antibody detecting EPOR in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EPOR (Ala25-Pro250).

Target

EPOR, Erythropoietin receptor, EPO-R

Purification

Purified by antigen affinity column.

Accession

P19235

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Erythropoietin receptor (EPOR) is a ~55 kDa protein. Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

1. Zhan, H. et al. (1999) Protein engineering 12, 505-13. PMID: 10388848

2. Winkelmann, JC. et al. (1990) Blood 76, 24-30. PMID: 2163695

3. Jones, SS. et al. (1990) Blood 76, 31-5. PMID: 2163696

4. Middleton, SA. et al. (1996) The Journal of biological chemistry 271, 14045-54. PMID: 8662939

5. Syed, RS. et al. (1998) Nature 395, 511-6. PMID: 9774108

6. Kirito, K. et al. (2002) Blood 99, 102-10. PMID: 11756159

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EPOR polyclonal antibody at 1ug/mL on recombinant EPOR (Catalog No. HB152012).

References

Datasheet

Anti-EPOR Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-EPOR Polyclonal Antibody (HB152014)

Overview

Images

References

Datasheet

SDS

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