Anti-EPX Polyclonal Antibody (HY020014)

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Overview

Catalog No.

HY020014

Description

Anti-EPX Polyclonal Antibody (HY020014) is a rabbit polyclonal antibody detecting EPX in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Homo sapiens (Human), Mus musculus (Mouse)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EPX (Arg140-Thr715).

Target

Eosinophil peroxidase, EPO, EC:1.11.1.7, Eosinophil peroxidase light chain, Eosinophil peroxidase heavy chain, EPX, EPER, EPO, EPP

Endotoxin level

Please contact with the lab for this information.

Observed Molecular Weight

~55 kDa

Purification

Purified by antigen affinity column.

Accession

P11678, P49290

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
WB	1:500-1:2000
IHC	1:50-1:500

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Eosinophil peroxidase (EPX) is a ~81 kDa protein. Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-Eosinophil peroxidase polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse spleen lysate

References

Datasheet

Anti-EPX Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EPX Polyclonal Antibody (HY020014)

Overview

Images

References

Datasheet

SDS

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