Anti-ERN1 Polyclonal Antibody (HF537014)

Name: Anti-ERN1 Polyclonal Antibody
Brand: abinScience
SKU: HF537014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HF537014

Description

Anti-ERN1 Polyclonal Antibody (HF537014) is a rabbit polyclonal antibody detecting ERN1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ERN1 (Phe571-Leu977).

Target

Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, ERN1, Ire1-alpha, hIRE1p, Serine/threonine-protein kinase/endoribonuclease IRE1, Inositol-requiring protein 1, IRE1

Purification

Purified by antigen affinity column.

Accession

O75460

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) is a ~109 kDa protein. Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes.

1. Iwawaki, T. et al. (2001) Nature cell biology 3, 158-64. PMID: 11175748

2. Yoshida, H. et al. (2001) Cell 107, 881-91. PMID: 11779464

3. Liu, CY. et al. (2003) The Journal of biological chemistry 278, 17680-7. PMID: 12637535

4. Lisbona, F. et al. (2009) Molecular cell 33, 679-91. PMID: 19328063

5. Ali, MM. et al. (2011) The EMBO journal 30, 894-905. PMID: 21317875

6. Liu, J. et al. (2017) Nature communications 8, 14186. PMID: 28128204

7. Chang, TK. et al. (2018) Molecular cell 71, 629-636.e5. PMID: 30118681

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Tirasophon, W. et al. (1998) Genes & development 12, 1812-24. PMID: 9637683

10. Eletto, D. et al. (2014) Molecular cell 53, 562-576. PMID: 24508390

Note

For research use only.

Images

References

Datasheet

Anti-ERN1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-ERN1 Polyclonal Antibody (HF537014)

Overview

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References

Datasheet

SDS

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