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Anti-Escherichia coli pcnB/Poly(A) polymerase I Polyclonal Antibody (JN951014)

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Overview
Catalog No.JN951014
Species reactivityEscherichia coli
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Escherichia coli pcnB/Poly(A) polymerase I (Leu13-Ala465).
Target Poly(A) polymerase I, PAP I, 2.7.7.19, Plasmid copy number protein, pcnB, b0143, JW5808, Escherichia coli (strain K12)
Purification Purified by antigen affinity column.
Accession P0ABF1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Poly(A) polymerase I (pcnB) is a ~53 kDa protein. Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control.

Note For research use only.
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Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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